918搏天堂

Effect soflow-molecular-weight citrus pectin on the sensitivity of colon cancer cell lines to oxaliplatin WANGFeng，LIUHai-ying．Department of Gastrointestinal Surgery，the Affiliated Tumor Hospital of Guangzhou Medical College，Guangzhou510095，China

【Abstract】 Objective To compare the effect of oxaliplatin solo and the combination of oxaliplatin and lowmolecular-weight citrus pectin （LCP） on the proliferation of colon carcinoma cell lines in vitro， and investigate whether LCP would influence the sensitivity of these cells to oxaliplatin． Methods Human colon carcinoma cell lines HT29， HCT116， SW116， SW480 were subjected to oxaliplatin or oxaliplatin plus LCP in various

concentrations for 72 h， and the sensitivity of these cells to oxaliplatin was tested by cell morphology observation and activity testing by MTT． Results There was suppressing effects of different concentrations of oxaliplatin on human colon carcinoma cell lines HT29， HCT116， SW116， SW480． LCP could strengthen the inhibiting effect of oxaliplatin on human colon carcinoma cell lines． Conclusion LCP could increase the sensitivity of human colon carcinoma cell lines to oxaliplatin.

摘要  目的：比较奥沙利铂单药及联合低分子柑橘果胶（LCP）体外抑制结肠癌细胞的增殖效应，探讨LCP是否影响人结肠癌细胞对化疗药物奥沙利铂的敏感性。要领：差别浓度的奥沙利铂单药及联合LCP作用于对数生恒久的人结肠癌细胞HT29、HCT116、SW116、SW480，通过形态学视察、四甲基偶氮唑盐法（MTT）细胞活力测定等检测对人结肠癌细胞敏感性的影响。结果：差别浓度的奥沙利铂对结肠癌细胞的生长均有抑制作用，而差别浓度的LCP抑制作用不明显，LCP与奥沙利铂联相助用于人结肠癌细胞可增强奥沙利铂对人结肠癌细胞的抑制效果。结论：LCP能增加奥沙利铂对人结肠癌细胞的敏感性，为将LCP推向结直肠癌治疗的临床试验和提高结直肠癌疗效提供理论依据。